Diclofenac Sodium Assay Method 4 is based on the formation of an ion-pair complex between diclofenac sodium and methylene blue under alkaline phosphate buffer conditions. Diclofenac sodium is an acidic drug and exists mainly in ionized form in phosphate buffer…
Diclofenac Sodium Assay – Acidification–Extraction Method The acidification–extraction assay method for Diclofenac Sodium is a widely used analytical procedure in pharmaceutical quality control laboratories. This method is based on converting Diclofenac Sodium into its free acid form through acidification, followed…
Great scientists have played a major role in the growth and success of the pharmaceutical sector. Their discoveries helped develop life-saving medicines, vaccines, diagnostic methods, and safer drug regulations. The pharma industry today stands on the foundation created by these…
Step-by-Step Procedure 1. Prepare the Smear Take a clean, grease-free glass slide. Place a small drop of sterile water on the slide if using a colony from solid media.Mix a small amount of bacterial colony into the drop and spread…
Diclofenac Sodium Assay Method Methanol Dissolution and Titration Method Principle Diclofenac Sodium is dissolved in methanol and titrated with 0.1 M sodium hydroxide (NaOH). The amount of Diclofenac Sodium present in the sample is calculated from the volume of NaOH…
Method 1: Non-Aqueous Titration Method Principle Diclofenac sodium is titrated in a non-aqueous medium using 0.1 M perchloric acid. This Diclofenac Sodium Assay (Titration) method 1 is suitable because diclofenac sodium behaves properly in non-aqueous solvent systems. Reagents Required Procedure…
Since both standard and sample final concentrations are same, assay is calculated by direct absorbance comparison. Formula % Assay=Sample ODStandard OD×100\% \text{ Assay} = \frac{\text{Sample OD}}{\text{Standard OD}} \times 100% Assay=Standard ODSample OD×100 Substitution % Assay=0.4980.512×100\% \text{ Assay} = \frac{0.498}{0.512} \times 100 % Assay=0.5120.498×100 =0.97265625×100= 0.97265625 \times 100 =0.97265625×100…
Paracetamol Standard Preparation Step 1: Weighing Weigh 100 mg of Paracetamol accurately. Step 2: First Dilution Transfer the weighed Paracetamol into a suitable volumetric flask. Add 25 mL of methanol and dissolve it properly. Step 3: Make Up Volume Make…
CPM Assay / Estimation Reagents R1: Dilute HCl solutionAdd 12 mL HCl and make up to 500 mL with DM water. R2: n-Hexane R3: NaOH solution (1M)Concentration is not clearly mentioned in the image. Standard Preparation Test Sample Preparation Chlorpheniramine…
Reagent Preparation: R1: 5% w/v Copper Sulphate in water R2: Benzene: carbon disulphide mixture, 4:1 R3: Strong ammonia Standard preparation: 1. Weigh 100 mg standard. 2. Dilute to 100 mL with DM water. 3. Take 5 mL of this solution.…